Republican Gov. Phil Bryant said in a statement that he signed the law to "protect the religious beliefs and moral convictions of individuals, organizations and private associations of discriminatory actions by the state government or political dependencies".
The rule, which is called Law on Protection of Freedom of Conscience of the Government Discrimination has found the opposition of groups of gay, lesbian, bisexual and transgender (LGBT) as well as local business and Economic Council Mississippi.
The group defending the rights of gay Freedom for All Americans described the standard as "the worst piece of legislation anti-LGBT whole nation."
The law prevents the state government punish any church, organization or company that refuses to provide its services to people if it is a violation of their religious beliefs that marriage is the union of a man and a woman and concepts "man" and "woman" are immutable.
Several US states They have legislated in recent months controversial rules by invoking religious freedom, following the landmark Supreme Court ruling last year that legalized same-sex marriage across the country.
To Mississippi, the most controversial law was North Carolina, passed last month by Republican Gov. Pat McCrory and limiting anti-discrimination protections for members of the LGBT community.
The standard North Carolina led today to the multinational online payments PayPal to announce that leaves a business expansion planned in such a state that had given employment to some 400 people, considering that the law is contrary to the values and culture of the company.
Also companies like American Airlines, Apple, Bank of America, Facebook, Google, IBM, Microsoft, Twitter and Yahoo have positioned themselves against the standard of North Carolina.
Meanwhile, the Seattle Mayor Ed Murray, and the governor of Washington state, Jay Inslee, both Democrats, decreed today the ban on all public employees to conduct official trips to Mississippi if they are not "essential" while the law is in force.